SYNTHESIS OF FATS ACCOMPANYING INTESTINAL ABSORPTION 37 
The results of this experiment shows that the formation of free acids is greater 
throughout in the case of pancreatic tissue than in that of the intestinal mucosa. The 
presence of dextrose apparently increases slightly the amount of free acid liberated, 
but has no action in producing any synthesis of neutral fat. 
Experiment 5. Since it might be supposed that in the preceding experiments 
the soap was hydrolysed by the water or saline used as a solvent, and not by any 
active constituent of the tissues extracted, a series of controls was carried out in which 
the same percentage of soap was dissolved, as follows : — 
No. 1. Distilled water, 40 c.c. + sodium oleate, cr8 gramme + glycerine, 0*4 
gramme. 
No. 2. Normal saline (0-75 per cent.), 40 c.c. + sodium oleate, cr8 gramme, 
+ glycerine, o - 4 gramme. 
No. 3. Solution of Na 2 Co 3 (o - 2 per cent.) 40 c.c. + saturation with carbon- 
dioxide + <0'8 gramme of sodium oleate + 0*4 gramme of glycerine. 
No. 4. Oxalated pig's plasma, 40 c.c. + sodium oleate, o - 8 gramme + 
glycerine, 04 gramme. 
All four flasks containing these respective solutions were placed in the incu- 
bator at 36° C. 
Examined as soon as they had attained the temperature of the incubator, 
No. 1 was found to be completely dissolved to a clear solution ; Nos. 2 and 3 were 
opalescent and contained a good deal of undissolved oleate ; No. 4 was clear, but 
contained a small amount of undissolved oleate. 
Examined four hours later, the appearance presented to the eye was much the 
same as at the previous examination, while microscopic examination showed no trace 
of fat globules in any of the four, merely fine amorphous granules were present in 
Nos. 2 and 3. 
Examined forty-six hours after the commencement of the experiment, during 
all of which interval the four flasks had been kept at a temperature of 36 0 C, Nos. 
1 and 4 were found to be perfectly clear solutions without a trace of cloudiness or 
precipitation, and showing a clear field under the microscope. Nos. 2 and 3 were 
opalescent and contained a sediment, but when examined by the microscope showed 
no trace of oily globules, and there was no surface layer of oil. 
Hence these controls clearly demonstrate that the formation of oleic acid 
observed in the previous experiments was due to some hydrolytic agent present in the 
extracts of the tissues. 
Series 2 
In the previous series of experiments, extracts free from cells were employed, 
and attention was now turned to the products obtained when the cells were present, 
along with the same constituents as in the previous series. 
