SYNTHESIS OF FATS ACCOMPANYING INTESTINAL ABSORPTION 41 
The results are given in the following table : — 
! Total ethereal 
residue 
Oleic acid 
Olein 
Soap, etc. 
No. 
1 
0-8034 
o- 1 942 
0-0294 
0-5798 
No. 
2 
0-6168 
0-1885 
0-0368 
0-3915 
No. 
3 
0-5772 
0-1294 
0-0368. 
0-41 IO 
No. 
4 
0-0526 
0-0309 
0-02 I I 
o-ooi 6 
It is here obvious from column three that the olein present is exceedingly 
small, and when the amount present in the tissue itself, as shown in No. 4, is deducted, 
comes well within the limit of experimental error. Column four again demonstrates 
that the difference between total extract and free fatty acid found in all the previous 
experiments is due to dissolved soaps. 
Addenda to this Experiment 
No. 1. Controls with distilled water. — The effect of the processes used in the 
above experiment upon solutions in distilled water (a) of sodium oleate and glycerine, 
and (b) of sodium oleate alone, in identical strengths with those used in the experi- 
ment, was next tested, the same quantities of solution, period of digestion at the 
same temperature, and the same methods of extracting and titrating- being employed 
throughout. These controls serve also for the succeeding experiments of this series. 
The results were as follows : — 
Total ethereal 
extract 
Oleic acid 
Olein 
Soap 
No. 1 (oleate + glycerine) 
o-6i 52 
0-0704 
0-0147 
0-5301 
No. 2 (oleate alone) 
0-5746 
0-0760 
0-0294 
O-4692 
The results also show that the oleic acid formed in the case of the intestinal 
extracts was not due to the experimental procedures employed, and that the olein 
there found lies within the limit of experimental error. 
No. 2. Action of the intestinal extract after evaporation to dryness and extraction 
with ether. — As experiment had shown that boiling for five minutes (in No. 3 of the 
previous experiment and elsewhere), although it somewhat diminished the power of 
the extract to produce oleic acid from sodium oleate, still left a considerable amount 
of activity untouched, the residue of No. 4, weighing 0^57 8 6 gramme, to which no 
soap had been added, and which had not only been boiled but reduced to dryness 
and then extracted with ether so that it contained no fatty matter, was tested as to 
whether it still possessed any activity upon sodium oleate. For this purpose it was 
G 
