42 THOMPSON YATES AND JOHNSTON LABORATORIES REPORT 
extracted with 40 c.c. of distilled water, and o - 8 gramme of sodium oleate was now 
added, the mixture was digested at 3 6° C. for a period of seventeen hours, and then 
the determinations made as before with the following results : — 
Total ethereal 
extract 
Oleic acid 
Olein 
Soap 
0-5352 
0-2759 
o-o 1 47 
0-2446 
This experiment, therefore, shows that the substance causing the hydrolysis 
of soap into free fatty acid is not destroyed or removed by prolonged heating to 
boiling point, such as takes place in evaporating to dryness on a steam bath, nor by 
extraction with ether several times. 
Experiment 2. Digestion with intestinal cells (pig) in distilled water for a 
shorter period. (Interval two-and-a-half hours). 
The quantities taken, and experimental procedures were as in Experiment I, 
but the time of digestion was reduced from seventeen to two-and-a-half hours, the 
results were as follows : — 
Total ethereal 
extract 
Oleic acid 
Olein 
Soap 
No. 
1 
0-5588 
o- 1 664 
0-0073 
0-3851 
No. 
2 
0-3956 
0-1382 
o-o 1 47 
0-2427 
No. 
3 
°"5 8 34 
0-1382 
o-oooo 
0-4452 
No. 
4 
0-0432 
OX 1 69 
0-0294 
o-oooo 1 
The amounts of oleic acid here formed are almost as large as in the longer 
period of digestion, showing that equilibrium had practically been reached in the 
shorter interval of two-and-a-half hours. 
Experiment 3. Intestinal cells (pig) in 0*75 per cent, saline, quantities and 
procedure as in previous experiment, interval two-and-a-half hours. 
Total ethereal 
extract 
Oleic aciil 
Olein 
Soap 
No. 
1 
0-4620 
o- 1 269 
0-0147 
0-3204 
No. 
2 
0-3224 
o- 1 269 
0-007 3 
0-I882 
No. 
3 
0*2706 
°'°733 
0-0147 
o- 1826 
No. 
4 
0-0224 
0-0197 
0-0147 
o-ooco 1 
1. [t will be observed that oleic acid and olein slightly exceed total ethereal extract, this is due to experimental error. 
