168 THOMPSON YATES AND JOHNSTON LABORATORIES REPORT 
and multiplies by budding. Two peculiarities are, however, to be seen : firstly, 
in many cases delicate processes connect adjacent specimens of the organism ; 
secondly, the capsule is often thickened and forms a kind of ' halo ' round the central 
deeply-staining body of the cell. 
' In the nodules in lungs, liver, spleen, and kidneys, which are secondary 
to the growths in the peritoneum, in addition to the forms already described, spore- 
bearing forms are found. In these the capsule of the cell is much thickened, the 
chromatin of the cells breaks up irregularly, and portions are allowed to escape 
through dehiscences in the capsule. There is no regularity in the process, no 
simultaneous division of the cell contents into a definite number of spores, and 
no simultaneous shedding of the same. The spores are without capsule when 
shed, and are irregular in contour. They stain deeply with chromatin stains, and 
are finely granular.' 
II. THE METHOD OF EXAMINING THE GROWTHS 
The tumours, the examination of which is described in this paper, are seven 
in number. In regard to Nos. I and 6, I was not present at the operation, but the 
tumour was in my hands within an hour and within two hours, respectively, after 
removal, and was conveyed from the operating theatre to the laboratory in sterile 
coverings. With regard to the other tumours, I either removed them myself or was 
present at the operation. Immediately after removal, each was wrapped in sterile 
gauze, outside of which was placed one or more sterile towels. I then brought it to 
the laboratory, and, after sterilizing my hands and instruments, I removed the cover- 
ings so as to expose the deep surface of the mass ; the skin surface remained down- 
wards in contact with the sterile coverings. With a knife or spatula heated in the 
flame the surface was then seared, and incisions made into the tumour. Small 
portions were then removed and immersed in the fluid media or rubbed over the 
surface of the solid media. Scrapings of the cut surfaces were also taken with the 
platinum loop and inseminated in the same manner. 
With regard to the examination of the animals inoculated : immediately after 
being killed or as soon as possible after death, the abdomen was shaved and scrubbed 
for some minutes with lysol (4 per cent.) The peritoneal cavity was then opened, 
and either the whole or parts of the organs affected removed and placed in sterile 
petri dishes. The surfaces of the organs were then seared with the heated spatula, 
and incisions made into the lesions. From the latter, pieces or scrapings were re- 
moved and inseminated. All manipulations were carried out as rapidly as possible. 
The tubes were in all cases incubated at 37 0 C. 
I have employed the following media : — nutrient broth, nutrient agar, milk, 
glucose broth (4 percent.), glucose agar (1 per cent.), lactose agar (1 percent.), ascitic 
fluid, ascitic fluid broth, ascitic fluid agar, ascitic fluid glucose broth, human blood 
