CHARACTERISTIC ORGANISM ASSOCIATED WITH CANCER 
run together in clumps, and are faceted against each other. The protoplasm of the 
interior shrinks away from the capsule and presents various irregularities of shape. 
2. Method of Reproduction of Type A 
Both of the above described varieties exhibit a method of multiplication by 
budding (Plate XIII, Fig. i). In the case of variety i, the method is as follows: — The 
bud appears first as a slight bulging of the capsule ; this gradually becomes more 
distinct ; the interior of this projection appears to be homogeneous in structure. As 
it develops it assumes a spherical contour, and its capsule is derived from the 
unbroken capsule of the parent. When near to full formation an intracellular net- 
work is to be distinguished, and when the parent cell possesses scattered refractile 
granules, one or more usually appear in the bud. I have not been able to distinguish 
any process of karyokinesis of the parent cell nucleus. Separation of the bud goes 
on gradually ; it may be delayed until a series of three or more is formed. When 
young cultures are kept at the room temperature these chains are not formed, but 
single daughter cells are produced and separate off ; if such cultures are incubated at 
37 0 C. the process is active, and a single parent cell may exhibit two or more buds, 
forming simultaneously in different stages towards separation. In the description of 
variety i, it was stated that in older cultures many of the cells shewed an aggregation 
of refractile and deep blue stained granules at the centre ; this type of cell rarely 
shows bud development, but in buds produced from these cells there is a gradual 
division of this nucleus-like granular mass which progresses with the formation of 
the bud ; a portion eventually becomes completely separated off and passes into the 
bud. In the case of variety 2, the bud produced may possess either characters 
similar to those of the parent, or the characteristics of variety i. In the latter event, 
the process of formation is similar to that just described, except that the capsule of 
the bud is not simply derived by distension from the complete capsule of the parent ; 
the outer layers of the capsule of the parent cell are ruptured, and the bud is projected 
out with a covering derived only from the inner layer or layers. When this variety 
2 produces a bud resembling itself the process is essentially similar to the budding 
of variety I. The interior of the bud is granular, and takes a deep blue colour, and 
it may derive its capsule from the whole or from part only of the parent cell capsule ; 
in many cases the latter is so delicate that it can be distinguished only with difficulty. 
2a. On a certain Process observed in Glucose Broth Cultures of Type A 
When a fresh glucose broth culture of A is made by inseminating a particle 
of glucose agar culture, after twenty-four hours' incubation there are present forms 
which exhibit the following process : — At a single point in the capsule there is to be 
seen a minute dehiscence or micropyle, and through this the granular contents of 
the cell are being protruded. The cells which shew this process possess a well-marked 
