CHARACTERISTIC ORGANISM ASSOCIATED WITH CANCER 173 
Example 2. Glucose broth culture inoculated May 31 ; plated June 22 on 
glucose agar ; colonies of both small type and of the A type developed. Micro- 
scopically the glucose broth culture shows the presence of both types, the small 
type being abundant. 
These are the only two examples, as I have already stated, of the separation 
of a small cell type of organism from cultures of type A. On the other hand, I have 
had many examples of the development of a similar small cell type when cultures of 
A are inoculated into animals. 
I take one example of this from my previous paper : — 
1. Guinea-pig inoculated intraperitoneally with organism of A type. Post- 
mortem lesions found in peritoneum, spleen, liver, lungs, and kidney. In these lesions 
groups of organisms are seen, which consist of A forms surrounded by a small type 
of organism in large numbers. Such a group is shown in the photograph (Fig. 2, 
Plate XIV). 
The following examples are from the present series of observations : — 
2. Guinea-pig inoculated, April 9, 1900, with pure culture of A. Killed 
April 24. From the lesions a small cell type of organism isolated in pure 
culture. 
3. Guinea-pig inoculated, February 6, 1903, with pure culture of A. Killed 
February 17. From the lesions a small cell type of organism isolated in pure 
culture. 
4. Guinea-pig inoculated, February 10, 1903, with glucose broth culture of 
A. Killed March 6. From the lesions, colonies both of A and of a small cell 
type separated. 
5. Guinea-pig inoculated, June 4, 1903, with glucose broth culture of A. 
Killed J une 12. From the lesions glucose broth cultures show a few A forms, but 
the greater amount of the growth consists of a small cell type. These cultures were 
plated, and colonies of the latter alone developed. 
6. Guinea-pig inoculated, June 4, 1903. Killed June 16. From the lesions 
glucose broth cultures were recovered, which showed, microscopically, both A forms 
and a small cell type form. Glucose agar plates show numerous colonies of the latter 
after twenty-four hours' incubation at 37 0 C. After forty-eight hours a few A colonies 
also developed. 
This small cell type of organism, thus isolated in two instances from cultures 
of A in vitro, and in many instances from the lesions produced by the inoculation of 
A, shows constant morphological characteristics, and a mode of further development 
uniform in all cases. 
// is an organism identical with this in type and characteristics that I have isolated 
from the tumours examined. 
