CHARACTERISTIC ORGANISM ASSOCIATED WITH CANCER 175 
changes which it has shown have varied according to the circumstances attending its 
inoculation, and the following examples are illustrative : — 
1. Guinea-pig inoculated intraperitoneally with B culture from a carcinoma 
on February 6, 1903 ; cultures recovered February 16, showing an organism of larger 
average size than that inoculated and with a greater variety of shape (Fig. 4, Plate XIV), 
the shapes noted being spherical, oval, oblate-spheroid, club-shaped, and forms of 
great length, with club-shaped extremities ; some of the large spherical forms are as 
large as the specimens seen in cultures of A. 
2. Guinea-pig inoculated, April 6, 1903, with B culture from carcinoma. 
Killed May 4. Organism recovered of larger average size than that inoculated, and 
shews a mycelium-life development. This culture was inoculated intravenously into 
a rabbit on June 2, and recovered from the blood on June 5 by introducing '5 c.c. of 
blood in 2 c.c. glucose broth. In these cultures the mycelial growth was marked, 
the individual organisms forming the mycelium were of large average size, and the 
largest shewed the characteristics of type A. A was not, however, isolated as a 
separate growth from the cultures. 
3. Guinea-pig inoculated, November 27, 1902, with culture from carcinoma 
of type B. Killed January 1, 1903. From the lesions there were recovered cultures, 
which consisted in part of type B of larger average size than in the injected culture, 
but also abundant growth of typical type A forms. From these cultures both 
B colonies and A colonies were isolated on glucose agar plates (Fig, 2, Plate XIV). 
The changes which have been observed in the two types A and B, and the 
relations between the two types, in so far as they have been traced, may be diagram- 
matically shewn. 
A 
I. A type. 2. (?) Intermediate form from which B develops. 3. B type, and 4B. 
B as modified in animals. 5. Form resembling A, seen in 4 and 4.A cultures. 
2A. Budding form. 3A. Bud separated, which resembles 5. 
In regard to this diagram there are two stages shewn which require comment, 
2 and 4 (a and b). To take the latter first, it has not been shewn that B in its 
