CHARACTERISTIC ORGANISM ASSOCIATED WITH CANCER 
Nutrient agar. Scanty white growth in stroke cultures, becoming slightly 
yellow in old cultures. 
Nutrient broth. Slow development of growth, which first appears after three 
days as a slight sediment. 
Glucose broth. Abundant growth alter twenty-four hours' incubation, partly 
as sediment and partly suspended. Older cultures, three days, shew plentiful 
sediment growth, with supernatant fluid almost clear. 
Type B 
Glucose agar. Grows as a whitish-yellow translucent streak along needle 
track on sloped medium, with discrete spherical colonies alongside streak in some 
cases. Culture is extremely viscid, and, in some cases, can be peeled off the surface 
like a membrane. 
Glucose gelatine. In stroke cultures grows as a pearly-white streak ; in stab 
cultures a feather-like growth along the needle track. No gas formation. Culture viscid. 
Potato. Whitish-yellow ribbon-like growth ; very viscid. 
Glucose broth. In most cases grows readily ; in twenty-four hours, at 37 0 C, 
causes a general cloudiness in the medium ; after three days there is also slight 
sediment growth. 
Both types of organism were cultivated in a 2 per cent, solution of glucose 
in ordinary tap-water. Type A developed readily, type B scantily. In neither case 
did alcoholic fermentation take place. 
IV. THE HISTOLOGY OF THE GROWTHS EXAMINED AND 
THE CULTURE RESULTS OBTAINED FROM EACH 
1. On April 5, 1900, a tumour from the breast of a young woman was kindly 
sent to me by Mr. F. T. Paul. Sections showed a highly cellular carcinomatous 
growth ; the cells of large size in masses and columns ; there was very little fibrous 
over-growth between the cell masses. 
The tumour was examined in the manner described in Chapter II ; the media 
employed were ordinary nutrient broth and nutrient agar. After three days' incuba- 
tion, slight sedimental growth was seen in two of the nutrient broth tubes. This 
consisted of organisms with the following characters : groups and masses of spherical 
forms with a fine intercellular faintly staining fibrillar material between the individual 
specimens ; the latter varied in size from that of an exceedingly minute granule to a 
cell with a diameter of about 4//. In dried coverslip preparations the organisms 
stained readily with methyl violet ; the smaller specimens deeply, the larger less 
intensely. 
Subcultures were taken from these in glucose broth and nutrient broth and 
on to nutrient agar, glucose agar, blood serum, and nutrient gelatine ; no growth 
Y 
