i"8 THOMPSON YATES AND JOHNSTON LABORATORIES REPORT 
developed on the solid media, but further cultures were obtained in the nutrient 
broth, and to a less extent in the glucose broth. These subcultures were of the 
same type as the primary cultures, except that the size of the organisms did not vary 
to such an extent ; the proportion of the minute to the largest specimens in the 
original cultures was about i : 15 ; in the subcultures about 1 : 8. 
Only one series of subcultures of the growth was obtained, and the organism 
thus rapidly lost vitality in the media employed. 
Inoculation experiments were made into guinea-pigs and dogs. The histology 
of the lesions thus produced will form the subject of a later communication, and I 
confine myself here to the facts which have to do with the life of the organism. 
On April 9, a guinea-pig was inoculated intraperitoneally with primary broth 
culture, and on May ri, a second was similarly inoculated with subculture from 
this. Both animals were killed, the first on May 1, the second on June 8 ; in 
both cases nodules were present, distributed generally throughout the peritoneal cavity, 
and in the spleen, liver, lungs, bronchial, and mesenteric glands. From these nodules, 
endeavours were made to obtain cultures, and from those in the animal killed on 
May 1, cultures grew on glucose -agar, but none were obtained from the second animal. 
The cultures obtained from the first animal were identical in appearance with that 
injected. 
On further injection of these cultures, no lesions resulted in guinea-pigs, and 
a similar negative result was obtained with all subcultures of the original culture, with 
the exception mentioned above. 
The nodules on the peritoneal surfaces just described were broken up with 
pestle and mortar in normal saline solution, and injected through a series of animals, 
as shewn in the following table : — 
Guinea-Pig i 
(Inoculated, April 9. Primary 
culture. Killed, Mai; i). 
Nodules. 
Guinea-Pig 2 
(Inoculated, May 11. Primary 
culture. Killed, June 8). 
Nodules. 
Guinea-pig. Inoculated, May 1. 
with emulsion. Killed, May 
28. Nodules. 
I . 
Dog. Inoculated intraperitone- 
ally, Ma)' 28. Killed, June 
29. Nodules omentum. 
Spleen and liver. 
I 
Guinea-pig. Inoculated June 29. 
Emulsion. Killed, August 8. 
Nodules omentum. Liver, 
spleen. 
Guinea-pig. Inoculated, May 1, 
with emulsion. Killed, June 
7. Nodules. 
I 
Guinea-pig. Inoculated, 
June 7. Died, June 16. 
Nil. 
Guinea-pig. Inoculated; 
June 7. Died, June 19. 
Nil. 
Dog. Inoculated in mammary 
gland, August 8. Nodules 
in mamma and spleen. 
