CHARACTERISTIC ORGANISM ASSOCIATED WITH CANCER 179 
Thus the nodules produced in the guinea-pig inoculated on April 9, 1900, 
and subsequently in another animal on May 1 1, were transplanted through four other 
animals in series. In each case, attempts were made to recover cultures from these 
nodules, but unsuccessfully. 
Scrapings from the peritoneal nodules in guinea-pigs were examined fresh in 
hanging-drop preparations ; there were seen in these, two types of cells which were 
distinguishable from the connective tissue and blood cells. The one type was of 
large size, an average of about 5^ with distinct capsule, and containing in the interior 
one or more refractile granules ; the second type was a small spherical organism 
about the size of a minute coccus. 
2. On November 24, 1902, I obtained a tumour from the breast of a 
pregnant woman, aged thirty-three ; she was five months advanced in pregnancy, 
and the growth was of rapid development. The parenchyma of this carcinoma con- 
sisted of large cells with large oval nuclei, arranged as branching columns in a 
fibrous stroma ; in the interstices of the stroma were numerous infiltrating round 
cells. In parts adjacent to the carcinomatous growth the breast shewed a condition 
of mastitis, the fibrous stroma being increased and infiltrated with round cells in 
large numbers, the ducts and alveoli were dilated. The media used in the examina- 
tion of this tumour were milk and glucose broth ; the method of examination was 
that described in Chapter II. Within forty-eight hours there was a general turbidity 
in most of the tubes of glucose broth ; microscopic examination shewed this to 
consist of organisms of type B, as described in the preceding chapter, that is to say, 
spherical organisms of very varying size, in masses and groups, with a considerable 
amount of filamentous material connecting the individual specimens in the masses. 
Inoculations of these cultures were made into guinea-pigs as follows — five cultures 
being employed : — 
Inoculation 
Killed 
Result 
a. 
November 27 
December 
3° 
Nil 
b. 
November 27 
January 1 
Lesions 
!>. 
December 8 (dog) . . . 
March 17 
Lesions 
c. 
November 27 
December 
30 
Nil 
d. 
December 3 
January 5 
Nil 
c. 
December 
January 5 
Lesions 
Thus of five cultures two only produced lesions in guinea-pigs on intra- 
peritoneal injection ; the inoculation of the dog was made into the mamma. 
