i8o THOMPSON YATES AND JOHNSTON LABORATORIES REPORT 
From the three animals, cultures were obtained as follows : — 
1. Guinea-pig. Culture types A and B. 
2. Dog. Culture type B. 
3. Guinea-pig. Culture type A. 
The culture marked b in the table above was further injected into animals as 
follows : — 
Animnl 
Inoculated 
Killed 
Result 
I . 
Guinea-pig 
January 26 
Februar)' 4 
Nil 
2. 
do. 
Februar) - 6 
February ] 6 
Lesions 
3- 
do. 
February 6 
Februar)' 1 7 
do. 
4- 
do. 
February 6 
Februar) - 1 9 
do. 
5- 
do. 
Februar) - 2 3 
March 27 
do. 
6. 
Rabbit 
'February 2 3 
April 27 
Nil 
From the animals, 2, 3, 4, and 5, in only one case were cultures recovered, viz., 
number 2. These presented certain peculiarities ; there were present organisms of 
type A, also in large numbers, rod-shaped, club-shaped, and ovoid forms (Fig. 4, Plate 
XIV), and thirdly, forms corresponding to type B. A second peculiarity of these cul- 
tures was that in subcultures the organisms belonging to type A disappeared, also the 
involution (?) forms and the growth consisted exclusively of organisms belonging to 
type B. 
The inconstancy of the results obtained in the inoculations of trie-cultures in 
this case are remarkable. As seen in the second table, animals inoculated with the 
same culture, and even on the same day, gave contrary results. The only explanation 
for this inconstancy is the varying susceptibility of the animals used. 
3. On March 4, 1903, I removed the lett breast of a woman, aged forty-two. 
The whole breast was indurated. Above and to the outer side of the nipple was a nodule 
the size of a walnut, ill-defined. The right breast was also indurated and slightly 
tender. On microscopical examination the nodule referred to shewed a carcinomatous 
structure with branching columns of epithelial cells, the rest of the breast shewed 
marked hypertrophy of the interstitial fibrous tissue, with masses of inflammatory 
leucocytic infiltration. The investigation of the tumour was carried out in the 
manner described in Chapter II. In this case inoculations were made on to sloped 
glucose agar alone, by rubbing pieces of the tumour over the surface of the medium. 
Often tubes thus inoculated, after forty-eight hours' incubation at 37 0 C, growth was 
plentiful in two, scanty but present in the rest. 
