CHARACTERISTIC ORGANISM ASSOCIATED WITH CANCER 181 
The growth in all tubes was of type B, with the variations of shape marked, 
the shapes noted were spherical, oval, club-shaped, and spindle-shaped ; the organism 
grew in clumps and masses, and in some or these club-shaped forms were arranged 
in a rosette-like manner. 
The vitality of this organism in subcultures proved very limited, and glucose 
broth subcultures were obtained from one of the glucose agar cultures alone. These 
subcultures were injected into animals as follows : — 
Guinea-pig subcutaneously 
March 9 to March 27 
Nil 
Guinea-pig subcutaneously 
March 9 to April 27 
Nil 
Guinea-pig intraperitoneally 
March 10 to March 13 
Lesions 
Rabbit intraperitoneally 
March 23 to April 28 
Nil 
Guinea-pig intraperitoneally 
April 6 to May 4. 
Lesions 
Thus of two guinea-pigs inoculated into the peritoneal cavity, one, inoculated 
with a culture five days old, died in three days ; the second, inoculated with a 
culture thirty-three days old, was killed twenty-eight days later. 
On examination of the first, general peritonitis was found with yellowish-white 
nodules in the spleen and liver ; on examination of the second, similar yellowish- 
white nodules were found in liver and spleen, but no peritonitis. Cultures were 
recovered from both these animals ; those recovered from the first resembled the 
inoculated culture, but the club-shaped forms were more numerous, and, except that 
the average size was smaller, it was also similar to the cultures described on p. 175 
recovered in case 2 from the peritoneum of a guinea-pig. 
The cultures recovered from the second of the guinea-pigs was of type B as 
described. 
In the case of the first animal, the cultures recovered were of extremely low 
vitality, and further propagation was not possible, while those from the second 
animal grew readily. It was, therefore, possible to follow certain morphological 
changes which took place in these subcultures. Those on glucose agar were of the 
usual B type, but those in glucose broth developed a mycelium-like growth of inter- 
lacing branches, the branches being composed of : individual organisms of shaped 
ovoid, spherical and oblate, jointed together ; this mycelial growth is described in 
Chapter III, p. 175, and the separation off* of spherical forms resembling type A is 
there noted. 
4. On March 30, 1903, the patient above referred to as case 2, was again 
operated on for a recurrence of the growth. From the material removed, inoculations 
were made in the usual manner on glucose agar and into glucose broth. Growth 
