TRYPANOSOMIASIS EXPEDITION TO SENEGAMBIA 
47 
Note by Dr. H. E. Annett 
The Results of Inoculation of Blood from the European Case, H.K. 
(vide p. 9) 
Tame Rats. — White, black and white. Injected intraperitoneal^ with about o - 2 c.c. peripheral 
blood diluted with normal saline solution ; in some twenty-five per cent, only was it possible to 
demonstrate parasites. The parasites first appeared in the peripheral blood in from fifteen to twenty-five 
days after the inoculation, and their presence continued for three or four days, apparently without any 
significant rise in temperature. The animals lived for periods varying from two to four months or 
longer, and occasionally only during this time could parasites be found. Usually on post-mortem 
examination they also seemed to be absent, in occasional instances only were they present in the heart's 
blood. 
Tame Mice. — White, brown, black, etc. Parasites were never seen after injection. 
Rabbits and Guinea-Pigs. — Seemed to be refractory. 
Monkeys {Rhesus). — Seem to be all susceptible to intraperitoneal infection. Parasites can be 
demonstrated in the peripheral blood only after the twelfth day after infection, their first appearance 
being preceded by a rapid rise in temperature, followed by a more gradual fall during the next three days. 
The number of parasites per cover-glass preparation varied during this period from eight to ten in 
number, occasionally more, and then gradually disappeared. A similar phenomenon occurred at intervals 
of five to ten days, the animal gradually losing weight until death occurred in from six weeks to three 
months. In one case, post-moriem, numerous parasites were seen, but in others none were found. 
Monkeys were inoculated at different periods during the time which K. spent in England, 
including two inoculations with blood two days before death, one of which died after three months' infec- 
tion ; the other shewed few parasites at times, and appeared very ill and emaciated on two occasions, but 
eventually recovered, and now, after a period of nine months, is health}', and no parasites can be found 
in blood, either microscopically or by inoculation into rats. 
In monkeys no such symptoms as are described in human cases occur, beyond the occasional 
rises in temperature (during which parasites are present in peripheral blood) and the emaciation. 
Notes on Post-Mortems of Experimental Animals 
Experiment XLIX — 
Post- Mortem of Guinea-Pig 
jiutopsy. — Died during the night. Body emaciated, some slight yellow-coloured fluid in pleural 
and peritoneal cavities, slightly turbid in the latter. 
Heart. — Normal ; right side contained a dark blood clot. 
Lungs. — Showed patch}' congestion marked in right lower lobe, otherwise normal. 
Liver. — Large, and slightly congested. 
Spleen. — 3-5 x rj x o-p cm. Slightly injected. 
Stomach. — Some congestion along greater curvature. 
The duodenum for about 3 cm. was much thickened and deeply congested ; about I cm. from 
pylorus it was adherent to liver and a coil of small intestine. In this situation three ulcers were found, 
one large, with a slough}' base ; this had almost perforated. In small gut Peyers patches were slightly 
thickened and inflamed. 
Kidneys. — Slightly injected. 
