14 
Anopheles On the following day (August 26th) the Colonial Surgeon 
tutT* 3 m * (Dr. P rout) and Dr. Berkeley found Anopheles larva? (mingled 
with those of Culex) in a tub of water standing in a yard at the 
Sanitary Office. With one exception, this was the only instance 
in the course of our investigations in which Anopheles was found 
breeding elsewhere than in a road-side puddle or ditch. 
Further From August 29th onwards, we made fresh discoveries of 
of Anopheles Anopheles larva? almost every day in various parts of Freetown, 
larva?, where the ground is flat, but especially in the district known as 
Bambura Town, to the north-east, and in another to the south- 
west of Tower Hill. Others were found on the Kissy Road, 
while a pool of muddy water in an open drain outside the Post 
Office produced several batches of larva? in succession. In most 
cases the larva? were in stagnant puddles, varying in area from a 
foot to several feet, at the sides of the streets, but many were met 
with in the still water in little bays at the sides of slowly run- 
ning shallow ditches. Whether the water was clear or muddy 
seemed to make no difference to the larva?, but green alga? (on 
which they feed) were nearly always present. In some of the 
. puddles tadpoles were numerous. 
I kept a number of the larva? for the purpose of obtaining the 
perfect insects, which proved to belong to the same species 
(A?iopJieles costalis, Lw.) as those found at Wilberforce. 
Anopheles It may be of interest to mention that shortly after our 
under' Dg discovery of the breeding-places of Anopheles in the streets of 
similar Freetown, I received from Dr. H. Strachan, Colonial Surgeon 
Lagos. 1011 a a ^ Lagos, specimens of the same species of Anopheles caught by 
him at that place, accompanied by the information that he had 
found the larva? in puddles left in the streets after rain 
Dr. Strachan had been a fellow passenger of ours on board the 
" Fantee," and had taken a great interest in our work and its 
possible results. 
It thus appears that the conditions in Lagos are very similar 
to those in Freetown, but for further information on this point 
we must wait for Dr. Fielding-Ould's account of his investiga- 
tions. 
I) H-f:ctions Concurrently with our search for breeding-places of Anopheles, 
' ' ' ' Major Boss every day continued his dissections of the adult 
Wilberforce. 
