54 
Research Bulletin A T o. 1 
Table 16. — Inoculation experiments with the White Ohio tubers 
by placing the inoculum on the surface after removing the 
skin of the tuber. 
No. of 
inoculations 
No. of 
successful 
inoculations 
Medium 
used 
Temperature 
Humidity 
30 
30 
6 
6 
30 
30 
o 
o 
0 
0 
18 
4 
Yes 
No 
Yes 
Yes 
Yes 
No 
25 to 27° C 
25 to 27° C ... 
25 to 27° C 
25 to 27° C 
8 to 10° C 
8 to 10° C 
Saturated air 
Saturated air 
Over H 2 S0 4 
Saturated air 
Saturated air 
Saturated air 
Here again it is evident that the use of medium with the 
fungus gives a greater number of successful inoculations, par- 
ticularly at lower temperatures and higher relative humidity. 
Table 17. — Unsuccessful inoculation experiments with White 
Ohio tubers in cases in which the fungus was applied, with 
and without medium, on the unbroken surface at the places 
indicated. 
Method of 
inoculation 
No. of in- 
oculations 
~' No. of 
successful 
inoculations 
Medium 
used 
Temperature 
Humidity 
Eye surface 
30 
0 
Yes . . . 
25 to 27° C. 
Saturated 
air 
Eye surface 
30 
0 
No... 
25 to 27° C. 
Saturated 
air 
30 
0 
Yes . . 
8 to 10° C. 
Saturated 
air 
Eye surface 
30 
0 
No ... 
8 to 10 C. 
Saturated 
air 
Lenticel surface . 
30 
0 
Yes . . . 
25 to 27° C. 
Saturated 
air 
Lenticel surface . . 
30 
0 
No.... 
25 to 27° C. 
Saturated 
air 
Lenticel surface . . 
60 
0 
Yes . . . 
8 to 10° C. 
Saturated 
air 
Lenticel surface . . 
30 
0 
No... 
8 to 10° C. 
Saturated 
air 
Stem-end surface 
5 
0 
Yes.. 
25 to 27° C. 
Saturated 
air 
Stem-end surface 
5 
0 
No... 
25 to 27° C. 
Saturated 
air 
Stem-end surface 
5 
0 
Yes . . . 
8 to 10° C. 
Saturated 
air 
