20 Nebraska Agricultural Exp. Station, Research Bui. 11 
the three grades — (1) original, (2) large, and (3) small. The 
ratios for the moisture-free weight of the unselected seed to the 
large and small seed were respectively 100:127 and 100:85, while 
the ratios for the total sprout value were 100:123 and 100:88 
respectively. This would indicate a rather close relationship 
between the size of the seed and its sprout value. 
Table 6 — Summary of the sprout value determinations for three 
distinct grades of all wheat varieties tested in 1913 and 191 
Kind of seed tested 
Relative 
weights of 
100 seeds 
Relative sprout values of 100 seeds 
(moisture-free) 
Stem growth 
Root growth 
Total veg- 
etative growth 
Per cent 
Per cent 
Per cent 
Per cent 
1913 CROP 
Unselected 
100 
100 
100 
100 
Large^ 
126 
122 
136 
126 
SmalP 
80 
82 
97 
85 
1914 CROP 
Unselected 
100 
100 
100 
100 
Large2 
127 
118 
137 
123 
Small3 
89 
94 
85 
91 
AVERAGE 1913 AND 1914 
Unselected 
100 
100 
100 
100 
Large2 
127 
120 
137 
125 
Small3 
85 
88 
91 
88 
iThese data are calculated from tables 2 and 3. 
2Seed classes indicated in tables 2 and 3 as "large" and "large plump" seed are here averaged 
in one group designated as "large" seed. 
3Seed classes indicated in tables 2 and 3 as "small" and "small plump" seed are here averaged 
in one group designated as "small" seed. 
LOSS OF SEED SUBSTANCE THRU RESPIRATION 
The sprout value determinations given in tables 2 and 3 
indicate that an actual loss of seed substances occurred which 
was not recovered in either the vegetative growth or the inert 
seed residue. A respiration test was made in triplicate which 
partially accounts for this loss. 
Apparatus was set up as shown in figure 5. Two hundred 
small plump Turkey Red wheat seeds were planted in the 20- 
liter, light-proof bottle No. 1, containing a small quantity of 
nonnutritive quartz medium. The seed was watered with 
distilled water from bottle No. 2 and the air was syphoned off 
thru bottle No. 3. U-tubes Nos. Ai, A2, and A3 contained KOH 
which extracted all CO2 from the air passing thru them. U-tubes 
