Relation of Size of Seed and Sprout Value to Yield 
21 
Bi, Bo, and B3 contained H2SO4, which extracted all moisture 
from the air. The U-tubes Ai and A2 prevent external atmos- 
pheric CO2 from entering the wheat bottle No. 1. U-tube 
Bi prevents any moisture reaching U-tube No. A3 which extracts 
and retails the CO2 liberated from the sprouting wheat in bottle 
No. 1. Some water is formed by chemical action when the CO2 
combines with the KOH in U-tube No. A3. This moisture is 
taken up by the H2SO4 in U-tube No. Bo. Thus the difference 
3 Bs Bo A3 Bi 1 A2 2 Ai 
Fig. 5 — (1) Light proof bottle in which seedlings were grown in a nonnutritive 
quartz medium. (2) Bottle of distilled water for watering seedlings. 
(3) Bottle of water for syphoning off air. (Ai, Ao, A3) U-tubes containing 
K O H for extracting CO2 from air. (Bi, B2, B3) U-tubes containing H2 
SO4 for extracting moisture from the air 
between initial and final weights of U-tubes A3 plus B2 indicates 
the amount of CO2 given off by the wheat seedlings as the result 
of respiration during germination and growth to the stage where 
all reserve food stored in the seeds has been translocated. The 
U-tube B3 removes all moisture from any possible back-flow of 
air from bottle No. 3. 
The seedlings were grown in bottle No. 1 entirely from the 
reserve food within the seed until the supply was exhausted and 
further growth terminated. Under the temperature conditions 
of this test a period of 14 days was required to exhaust this 
reserve food supply within the seed. The seedlings were then 
carefully washed from the sand, and the dry matter in the root 
