36 
(Grain XX daily)]. The attacks last five to nine days. One 
•or both eyes may be attacked, and not uncommonly do they 
alternate. One eye may cease to have attacks while the 
other continues. The experiments here recorded were made 
with the idea of transmitting the disease to other horses. 
They were only partially successful. 
During eight months the affected animal referred to us 
had six attacks of ophthalmia. The attack was observed on 
the first occasion, but during the second attack his anterior 
chamber was entered, the exudate aspirated and injected 
in the eye of a horse with apparently healthy eyes. The 
history of this second horse will be given later. The attacks 
of the first horse ranged from six to twelve days. Five of 
the six affected the left eye and one the right eye. In January, 
1910, the left eye was used for further inoculation and, follow- 
ing this traumatism, complete recovery never took place. 
The corneal scar left by the needle track almost disappeared, 
but an inferior anterior synechia formed and was followed 
by a spreading opacity of the cornea, much wrinkling of the 
iris and opacity of the depths. After the fourth attack in 
this eye it was completely blind. Material was obtained 
from this eye during its last attack, but it was merely serous 
fluid containing a few blood cells and epithelium, but no 
bacteria. 
In transferring the affection from this animal, the conjunc- 
tival sac was w^ashed with 1-5000 Bichloride of Mercury solu- 
tion and well rinsed with Salt solution. The anterior chamber 
was then entered with an aspirating needle and the exudate 
removed. This consisted of .4 cc. slightly turbid straw 
colored fluid containing a few shreds of lymph . Bacteriological 
cultures, moist and dry preparations were made from a part 
of this, while the remainder was introduced into the anterior 
chamber of the second horse. This animal's eye showed 
the efTects of the traumatism for eight days and then was 
normal save for a small opaque spot in cornea left from 
needle puncture. After 23 days a small patch of lymph 
collected in the pupil. This increased slowly, accompanied 
by lachrymation until the 27th day when a sudden and 
violent conjunctivitis arose. The lymph in the anterior 
