37 
chamber likewise suddenly increased and rapidly became- 
pus, forming a hypopyon. 
The conjunctivitis became purulent. The violent acute 
stage lasted five days and slowly subsided, leaving an ectropion 
with a densely injected bulbar conjunctiva, almost complete 
corneal opacity and an irregular contraction of iris, apparently 
due to several small synechise. The depths could not be 
seen because of the corneal condition. This stage of affairs 
remained during the rest of the animal's life, two months. 
He was permitted to live to see if an exacerbation of this 
chronic process or involvement of the other eye would appear.. 
Such not occurring in two months, he was killed and the eyes 
removed. 
Fluid removed from the left eye of the first horse when 
killed during the last attack, was injected into the anterior 
chamber of a third horse. This animal's eye received the 
operation well and the trauma had entirely disappeared 
when the animal died on the 8th day. 
Laboratory Examinations. 
From fluid removed from Horse Eye (No. 1) anaerobic 
cultures made on milk and blood serum, aerobic on blood 
serum, blood agar, glycerine agar ; smears were made directly 
from the fluid and coagula upon slides and stained as follows: 
Loefflers, Grams, Giemsa. In all there are very few recogniz- 
able bodies. They are R. B. C, polynuclears and a very 
few small mononuclear cells. In regard to micro-organisms 
three structures present themselves. A well staining Gram 
positive rounded end rod of fairly uniform size but tending 
to grow in pairs and stain rather irregularly with Loeffler 
and Giemsa. These forms are sometimes almost dumb-bell 
in that they are bipolar, or even seem to have a constriction 
in their centre. Another form is peculiar and cannot be said 
to be recognized as a bacterium. It is circular, of fairly 
regular size and contour and in many places looks like a 
very large coccus. In Loeffler 's stain it is colored deeply in 
the centre with a paler marginal zone and an unstained halo 
about it, which, however, is not like a capsule. In the Gram 
and Giemsa method it is deeply blue or purple with a refrac- 
