34 
Bacteriological observations were made upon cultures 
obtained from the intestinal mural lesions, the peritoneal 
exudate, the liver necroses and the hearts' blood in eleven 
cases. In seven cases I was able to isolate a motile rod 
like the B. Coli Communis, and in four cases a non- 
motile rod of the Bact. Aerogenes type. The former is 
quite similar to the B. scoticus (Migula) reported in 
grouse disease. Experiments upon the pathogenicity of 
these organisms are projected. 
Judging from reports and based upon the observations 
of Morse upon Grouse disease it would seem that the 
incubation period of the disease is about eight to ten 
days. However, one of the third lot of our birds died 
within three days of its arrival at this Garden, and 
therefore within three days of its exposure to the second 
arrivals; if it be correct that this second lot brought the 
disease and the third lot did not have it, it w^ould seem 
that the incubation period can be as short as three days; 
how long it may be is only suggested by the fact that 
some of the third lot did not die for three weeks after 
arrival and exposure. All of the Gambel's and scaled 
quail succumbed to the disease, but two of the 12 bob- 
white survived. It would seem that although these 
last birds probably introduced the disease, they still 
possessed more resistance than the others, for the second 
death among them occurred seventeen days after the 
first death. The epidemic as we have seen it here seems 
to be the same as Grouse disease of Scotland and as the 
Grouse disease in this country as reported by Morse 
(Bureau of Animal Industry Report, 109, May 18th, 
1907). 
The means of transmission of the disease is not exactly 
known, but it is in all probability by a pollution of the 
food, the water supply, or the ground. Since the lesions 
are so marked in the lower ileum, cecum and colon, a possi- 
ble transmission by cohabitation must not be entirely 
overlooked. There does not seem to be any means of 
limiting the epidemic in a flock by segregation or sacrifice 
of the infected birds, because symptoms are few and do 
not appear until shortly before death. Each bird would 
