49 
Examined December 13th. — Great White-Crested Cock- 
atoo. Roseate Cockatoo No. 3. 
Birds dying later — autopsy confirming diagnosis: — 
Red Vented Parrot (2424). No spiroptera. 
Rosehill Parrakeet. No spiroptera. 
As a result of this systematic examination we have 
learned : — 
First. — That we have in the garden 20 verminous birds 
out of a total of 136 examined — 14 per cent. 
Second. — The worms do not all kill quickly. Orange- 
winged Amazon was detected November 3d, 1911, three 
months ago. We must remember, however, that this is 
the off season for spiroptera, as shown by chart No. 2, 
and we do not now expect many deaths. For this reason 
it seems unwise at present to make any deductions as to 
how the expected 20 or 30 deaths next summer will be 
made up, whether from the 20 birds now isolated, or 
also from birds at the parrot house whose worms may be 
now developing. 
2. Life History of Spiroptera incerta. 
The work along this line has been disappointing. Ova 
from proventricular slime of a bird dead with spiroptera 
were placed in various nutrient media (bouillon, con- 
densation water of blood serum media), tap water and 
weak alkaline and acid solutions. These were kept at 
various temperatures. One series at room temperature, 
another at 37.5° and a third at the temperature of a 
bird's body (41°). They were examined daily. On the 
sixth day larval worms could be seen issuing from the 
eggs in tap water at room temperature. They did not 
hatch in the acid solution, but did appear sporadically in 
the other solutions at room temperature. The larvae 
