TAKEWO HEMMI 273 
produces at first a white, web-like growth over the surface of the twig as 
well as in the bark. In about two weeks after inoculation, thick globose 
masses of mycelium, where the pycnidia or the perithecia are to develop, are 
produced in large numbers all over the surface of the twigs. Here and there 
the creeping mycelium and the globose masses turn brownish-yellow, especial- 
ly on the cut surface. Only under moist condition in the dishes do the 
pycnidia thus formed push out very long red threads, composed of innumer- 
able pycnospores. If one observes closely the development of such globose 
masses of mycelium, it is seen that the stromata of the fungus are first pro- 
duced under the periderm and then the excessive growth of the mycelium 
takes place, rupturing the periderm. The pycnidium is formed either in the 
globose mass of mycelium or in the stroma under the periderm; while only 
under the periderm of the sterilized twig can the perithecia develop, having 
long necks which form the canals through such a mycelial mass. The globose 
masses are composed of the entangled mycelium and are not so compact as 
the stromata in nature. Unless careful observations be made, the develop- 
ment of the perithecia may be overlooked, for they are produced in the bark. 
The sterilized twig was the only medium on which I have succeeded in 
producing ascospores in the artificial cultures. 
(2) Bark-Decoction Agar. 
When streak cultures are made on bark decoction agar slants with the 
spores from a spore-horn, the mycelium begins to spread along the streak 
as a white or light brown weft and spreads rapidly toward the edge. The 
mycelium is apt to creep on the surface, and the aerial mycelium is scant. But 
in a week, at ordinary room temperature, the brown color begins to appear 
along the streak, and it broadens until the whole surface of the slant is cover- 
ed with such brown mycelium. After a while, the small bunches of the 
mycelium appear over the surface of the medium, and soon some of them turn 
into the compact stromata which produce numerous pycnospores, forming 
beautiful reddish masses. But I have not yet been able to get ascospores on 
