TAKEWO HEMMI 
28l 
growth on the liquefied medium. 
(4) On the medium containing 0.2 °/ 0 of tannic acid, the pycnospores are 
produced at about the ninetieth day after inoculation, and the spore develop- 
ment is not vigorous. 
(5) In the liquefied tubes, from 0.8 % to 2 °j 0 tannic acid, the growth 
of the causal fungus becomes less evident, generally appearing in floating 
patches, embedded masses, or lateral growth along the side of the tube. 
But it never makes a continuous coating over the surface of the medium. It 
generally fails entirely to make any growth at above 5 °/ 0 , and only in a few 
tubes of 5 °/ 0 did a very poor growth take place. Finally, at the highest 
percentages, 8 to 14, growth or even germination entirely ceases. 
(6) Valsa Mali and Valsa Paulowniae generally show a poor growth on 
these cultures, and spores have not been produced. In the high percent- 
ages of tannic acid, Valsa Mali shows an enfeebled growth sooner than does 
the causal fungus of this disease, since at 0.8 °/ 0 it makes comparatively little 
growth, which corresponds to the condition shown by the causal fungus at 
about 1.2 to 2 °j 0 . Valsa Mali generally fails to make any growth at above 
1.2 °/ 0 , or only a very poor growth is made in a few tubes up to 2 °/ 0 . At 
above 5 °/ 0 the growth or even the germination of Valsa Mali entirely ceases. 
Generally, the toxicity of tannic acid against Valsa Paulozvniae seems to 
be more conspicuous than against Valsa Mali. The culture solution con- 
taining tannic acid in which the Paulownia fungus distinctly shows its 
growth are only up to 0.2 °/ g , although up to 1.2 % m some tubes a very 
poor growth could be noticed. In the Petri-dish or Erlenmeyer's flask cul- 
tures with the same media, I obtained almost the same results. The results 
of this experiment are given in Table I. 
Table I. 
Table showing the different degrees of growth of the causal fungus and two 
other species of Valsa on the standard medium containing different percentages 
of the tannic acid. The 4- sign indicates the grozvlh of the fungus and its number 
