TAKEWO HEMMI 283 
From these experiments I obtained the following results : 
(1) All the media remain liquefied when citric acid is added, but some 
of them were at last solidified in consequence of the vigorous growth of the 
hyphae. 
(2) The growth of any of these fungi causes for the most cases no 
darkening of the media even when citric acid is added. 
(3) The causal fungus grows in all the cultures containing citric acid 
except 2 °/o, showing a more vigorous growth than in the check cultures. The 
cultures containing 2.0 % °f citric acid show a comparatively poor growth 
of the causal fungus and a darkening of the medium takes place in some 
of them. 
(4) Some of the cultures of this fungus containing 0.4 % of citric acid 
produce the pycnospores at about the 120th day after inoculation, and the 
spore development is conspicuously vigorous all over the surface of the 
medium. 
(5) The color of the mycelium of this fungus gradually turns from white 
to light yellow on the cultures containing citric acid, while on the cultures 
containing tannic acid it turns to dirty brown. 
(6) Although Valsa Mali grows in all percentages, its growth is general- 
ly poorer than in the case ,of the causal fungus ; and the spores are not pro- 
duced. 
Although Valsa Pauloivniae grows vigorously in all percentages, yet in 
the tubes containing 0.2 and 0.4% of citric acid its growth is most vigorous. 
Tlie cultures of the fungus containing 0.8, 1.2 and 2.0 % of citric acid 
produce numerous pycnospores at about the 1 20th day after inoculation, and 
spore development is also conspicuously vigorous, with the exception of 2% 
culture, in which the development is rather poor. 
The results of this series of experiments are given in Table II, with the 
same signs as in the preceding Table. 
