TAICEWO HEMMI 285 
(2) The medium containing tannic acid remains liquefied when 0.7 or 
1 .0 °/ 0 is added. 
(3) On all cultures containing tannic acid, the mycelium turns to a dirty 
brown color, while on checks it has remained colorless. 
(4) Check cultures containing no tannic acid show a vigorous growth 
of mycelium, which is, however, very loose, sending up the aerial mycelium. 
Spores have not been produced in these check cultures. 
(5) Cultures containing o. 1 % of tannic acid also show a vigorous 
growth of mycelium, but its entanglement is a little closer, and some of them 
produced numerous red masses of pycnospores at about the 50th day after 
inoculation. In this case, the spore production is more vigorous than in the 
cultures containing 0.4 % of tannic acid. 
(6) Cultures containing 0.4 % of tannic acid show the most vigorous 
growth of mycelium in this series of experiments, forming a close coating 
over the surface of the media. Some of them produced also numerous red 
masses of pycnospores at about the 50th day after inoculation. 
(7) The growth of hyphae on the cultures containing tannic acid seems 
to be slow at first, but later becomes more luxuriant, compared with the 
cultures without it. 
(8) Some of the cultures containing 0.7 °j 0 of tannic acid also show a 
vigorous growth of mycelium, but it takes a long time for the mycelium to 
appear and grow on the surface, due to the liquid of the medium ; and at last 
the growth of the fungus tends to form a more or less close coating over the 
surface. But in some of these cultures the fungus entirely failed to grow. 
(9) All cultures containing 1 .0 °/o of tannic acid fail to allow the growth 
of the fungus. 
The results of the second series of experiments are given in Table III. 
Table III. 
Showing the effect of the different percentages of tannic acid in the standard 
medium on the growth of the causal fungus. 
