TAKE WO HEMMI 291 
by Brefeld 3) in the case of germination of Valsa ceratophora Tul. 
The germinating hyphae are hyaline at first, about 3.2-6.0// in width, 
and often the old parts turn light brown or light yellow. In a drop of bark 
decoction, it was occasionally observed that some germinating hyphae stopped 
their growth when they reached about 32.0-80.0 ij. in length, swelling a little, 
and that soon the fine horn-like hyphae developed again from their swelled 
ends (PL X, Fig. 3.). 
In the experiments, I obtained pure ascospores from the perithecia on the 
natural host by letting them eject the spores on a slide. In the process of 
germination of the pycno- and ascospores, the various behavior of the hyphae 
and changes produced in their contents are very similar. The ascospores of 
our present fungus do not require a period of rest, but germinate directly 
after maturity, if placed under favorable conditions. 
c. Observations on the Hyphae in Drop Cultures. 
If the surrounding conditions are favorable, the hyphae started from 
the germ-tube nroceed with their growth in a radiating manner. But the 
rate of growth varies considerably with the temperature and the kinds of 
nutritient solutions; and in such an acid solution as fruit juice, the hyphae 
grow most actively. In the b:u k decoction, the growth is not so active, but 
the dense and short branching, which is said by many authors to be a young 
stage in the development of the pycnidium, is apt to be quickly produced. 
The growing hyphae are always hyaline, although their basal parts turn 
yellow or light brown ; and the hyphae are also not of uniform diameter, but 
vary, according to my observation, from 2.0 to 8.0 [i. 
Under the microscope, we often see the hyphae anastomosing in many 
ways ; and in consequence, they at last make the hyphal network. After more 
than ten days, it is not an infrequent sight to see them entangling in a mass. 
Occasionally I saw a substance being secreted on the tip of the hyphal branch, 
which is at first hyaline and at last turns grayish yellow or dirty yellow. But 
the nature of this secreted substance is not yet known to me. 
