TAK.EWO HEMMI 299 
was burned in addition to the same methods used in Experiment II. The 
experiment was based upon the idea that the fungus may first require the 
dead cells for the penetration of its germ-tubes. Upon such a hypothesis, 
infection experiments were performed by Adekhold with great success in 
the case of Valsa leucostoma. To keep it moist, I placed the pot under a 
bell-glass for one week. The results are given in the following table. 
Table XI. 
Inoculated bark 
Inoculum 
Results 
Smooth bark 
Pycnospores from culture 
T.enticel 
Pycnospores and mycelium from culture 
Cut and burnt bark 
Pycnospores from culture 
+ 
Cut and burnt bark 
Mycelium from culture 
+ 
New cut wound 
I Hseased bark 
New cut wound 
Ascospores from natural host 
New cut wound 
Pycnospores from culture 
New cut wound 
Mycelium from culture 
New cut wound 
Pycnospores and mycelium from culture 
The seedling which had been inoculated with the mycelium to its burnt 
portions suddenly wilted in about two weeks after inoculation, and the seed- 
ling which was inoculated with the pycnospores to a similar wound also 
suddenly wilted in about three weeks after inoculation. But even on the same 
seedling, the twigs above a burnt wound which was treated in the same way, 
with the exception that no spores were introduced into the wound, did not 
show any change for a long time. In the former case, the cork layer of the 
infected portion was raised in numerous little blisters. Sections through 
such a dead portion showed the tissues of the bark as well as the water ducts 
of the woody portion to be traversed by the mycelium. Under each blister 
was a compact mass of the mycelium, which is no doubt a young stage in 
the development of the stroma. Hut in no blisters were pycnidia developed, 
whose non-formation is chiefly due to the resistibility of the young host. 
