ON A NEW CANKER-DISEASE OF PRUNUS YEDOENSIS, etc. 
Experiments IV. 
As a result of the above experiments, it was necessary to try inoculations 
on ajlarge branch. On January 19th, 1915, a large branch of Prunus yedoensis, 
about four centimeters in diameter, was cut and put in water in the labora- 
tory ; and the water was renewed every day in order to prevent the multiplica- 
tion of micro-organisms in it. On about the 20th of February the branch was 
in full blossom. On the day when the branch was cut, it was inoculated with 
the fungus in various ways, and on the 13th of February the results were as 
follows : — 
Table XII. 
Inoculated portion 
I noculum 
Results 
(1) 
New cut wound with scalpel 
Ascospores from natural host 
Bark slightly sunken and gum 
flowed out 
Check 
■ 
No change 
(2) 
New cut wound with saw 
Pycnospores and mycelium from 
culture 
No change 
Check 
No change 
(3) 
Burnt wound 
Ascospores from natural host 
Bark sunken, gum flowed out and 
stromata developed 
Pycnospores and mycelium from 
culture 
Bark sunken, gum flowed out and 
stromata developed 
Check 
No change 
In these experiments, the wounds were kept moist by covering them with 
moist cotton, and then binding them up with paraffin paper for a week. 
In the first case, the portion around the inoculated point stopped its 
growth, forming an elliptical sunken spot ; and the gum was exuded from the 
inoculated wound. But in such a sunken area, the progress of the disease 
soon ceased, and no stroma of the fungus developed in it. But on the con- 
trary, the sunken area of the third case gradually increased its extent and the 
stromata of the fungus were developed sparingly upon it. When the branch 
was in full-bloom, thirty- five days after the inoculations, it began to wilt, 
showing a diseased appearance. In April, numerous small pustules of the 
fungus stromata appeared, scattered throughout the surface of such a dead 
