TAKEWO HE MM I ß OI 
branch, showing the symptom of a girdled branch. Similar experiments 
were also undertaken with smaller branches, and the same results were 
obtained. 
Besides these experiments, I also inoculated the same fungus on the 
branch of Prunus Cerasus, using the same method, but the results were 
negative. 
Experiments V. 
In this case, I used two comparatively aged potted trees of Prunus Miime 
which were at that time in full-bloom in a green house, and on each tree 
small burnt areas and slit wounds by a scalpel were made just before inocula- 
tion. In the injured portions of the one I inoculated the mycelium from a 
culture which was isolated from P. Mume, on February r2, 191 5. On another 
one I inoculated the mycelium from a culture which was isolated from Prunus 
yedoensis on the same day. To keep it moist, I placed the pot under a bell- 
idass for one week, and then it was placed for a long time in a warm labora- 
tory. 
On the first tree, the bark of an inoculated branch changed its color 
around the burnt portion, then gum flowed out, and at last many small pus- 
tules of the fungus stromata appeared rather scatteringly, while the checks 
indicated no change. The discolored portion of the bark gradually extended 
its area, which was sunken slightly, but up to the end of June the branch 
w as still in foliage, except a small twig which was branched near the infected 
portion. From July to September, the upper portion of the branch from the 
infected point died gradually, and the fungus stromata also produced scatter- 
ingly all over its surface. On the other branch which was inoculated in the 
slit wounds, the results were negative. 
On the second tree, the bark in the vicinity of both burned and injured 
portions on which inoculations had been made, first changed color, then gum 
flowed out, and at last the upper two twigs died, when there appeared also 
small pustules of the fungus stromata. The wilted flowers remained clinging 
