ON THE OCCURRENCE OF CREATININ IN LEGUMINOUS SEEDS. 
19 
EXPERIMENTAL. 
As the materials for the present investigation the following legumi- 
nous seeds were selected. 
1. Adzuki bean. Phaseobis radiains var. aitrca Prain. 
2. Black soy bean. Glycine Jiyspida Maxim. 
3. Yellow soy bean. 
4. Kidney bean. Phascolus vulgaris L. 
5. Green peas. Pisiivt sativum L. 
6. Horse bean. Vicia Faba L. 
The seeds were washed Avith water to remove dusts and other impuri- 
ties, dried and ground in the mill as usual. To remove fats and oils 
contained in the seeds, about double xolumes of ether were added to the 
ground preparation and allowed to stand a day under frequent shaking. 
The clear ether extract was then decanted and filtered. To the residue 
another portion of ether was added and after standing for a day under 
frequent shaking, it was filtered. The residue was then left at the room 
temperature until the smell of ether was no more appreciable. As the 
soy beans contained such a large amount of oil that the above process 
was not found satisfactory, they were extracted in the Soxhlet's apparatus 
^\■ith ether in the usual manner. 
The sample thus prepared was boiled in a large flask provided with 
reflux condenser, with strong alcohol, for several hours. The alcoholic 
extract was filtered hot, and the residue was pressed to remo\'e the extract 
well. The extract thus obtained was allowed to stand 24 hours, when 
some precipitates were found to be formed. They were separated by 
filration and the clear filtrate thus obtained was concentrated by evaporat- 
ing in a partial vacuum. As the solution became concentrated, some 
fatty substances seemed to separate out, the liquid was transferred to a 
porcelein basin and stirred with a glass rod under the addition of water, 
when fat-like substances adhered to the bottom of the basin as well as 
to the glass rod. The clear solution was decanted and evaporated on a 
