24 
K. OSIIIMA AND M. ARIIZUMI. 
Table HI. 
Sntiij) from 
Jaffe's 
Wey I's 
S a 1 k o w s k i ' s 
reaction 
reaction 
reaction 
A 1 1 • I 
Adziiki bean. 
+ 
+ 
+ 
+ 
+ 
]5hick soy bean. 
+ + 
+ 
+ 
+ 
■t- 
Yellow soy bean. 
+ 
+ 
+ 
+ 
Kidney Bean. 
+ 
+ 
+ 
Green peas. 
+ + 
+ 
+ 
-f- 
+ 
Horse bean. 
+ + 
+ 
+ 
+ 
+ 
From the results 
obtained, it is seen 
that 
in all 
cases the 
creatinii 
was precipitated with 
cuprous oxide and 
recovered 
again by h}-drogei 
sulphide. 
To the concentrated solution obtained in the above treatment, saturat- 
ed solution of zinc chloride and a little sodium acetate were added and 
the whole was allowed to stand several days. Within a few hours crystals 
began to form, and in a few days they were observed to have the 
characteristic appearance of Creatinin zinc chloride. The time rec|uired for 
crj'stal-formation and the forms of crystals are shown in the following. 
Adzuki bean, black soy bean and yellow soy bean. Crystalisation 
completed in 24 hours. Crystal-forms were radiating needles, crosses and 
clusters. 
Kidney bean. Crystallisation completed in 24 hours. Crystal-forms 
were radiating needles, crosses, cl Listers and plates. 
Green peas. Crystallisation completed in 48 hours. Crystal-forms 
were stars, rosettes and plates. 
Horse bean. Crystallisation comi^Ieted in 5 da}'s. Crystal-forms were 
radiating needles, crosses and clusters. 
It is thus seen that by separating Creatinin with cuprous oxide from 
the admixed impurities, the crystallisation could be effected much sooner 
than in the case of direct treatment. Even in the extract of soy bean 
with which we were unable to obtain the characteristic crystals of Creatinin 
zinc chloride by direct treatment, the crystallisation was completed within 
24 hours. In everj' case, the crystal-forms were very distinct and free 
