ON THE OCCURRENCE OF CREATININ IN LEGUMINOUS SEEDS. 
from impurities when seen under the microscope. 
Regaining of Creatinin. 
The regaining of Creatinin from Creatinin zinc chloride was undertaken 
in tlie following manner. 
The crystals obtained from the extract of Adzuki bean were separated 
from the mother-liquor by iiltration and well washed with strong- alcohol. 
The residue on the filter was dissolved in boiling water and filtered hot 
and the filtrate was allowed to recrystallise after concentration. The 
operation was repeated once more. The crystals thus obtained were 
white in color and when observed under microscope, they were somewhat 
oblong hexonal plates. They were dissolved in water and boiled with 
some freshly prepared lead h}'droxide, filtered, and the filtrate was con- 
centrated to a small volume. The concentrated solution gave Jaffa's 
reaction so distinctly that there is no doubt of the presence of Creatinin 
in it. Unfortunately from the lack of the material, other reactions could 
not be observed. 
The crystals of Creatinin zinc chloride from the extract of legumes 
other than Adzuki bean were so little that they did not allow further 
working up. 
Summary. 
1) The color tests, the formation of characteristic double salt of 
Creatinin zinc chloride and lastly, the regaining of Creatinin from the 
double salt, were applied to determine the presence of Creatinin in legumes. 
2) The presence of Creatinin was confirmed for the first time, in the 
seeds of Adzuki bean, black soy bean, yellow soy bean, kidney bean, 
horse bean and green peas. 
3) The amounts of Creatinin in the seeds of Adzuki bean, kidney 
bean and soy beans are apparently' in much larger quantity than in horse 
bean and green peas, though, its absolute amount seems to be very small. 
