ON THE CARBOHYDRATE GROUP IN YAM MUCIN. 
249 
with water. The filtrate was decolorized with animal charcoal and concen- 
trated to crystallization. When the syrup was kept over sulphuric acid for 
some time, tyrosin began to crystallize in characteristic delicate silky needles. 
It gave a beautiful red color with diazobenzensulphonic acid in the pre- 
sence of sodium hydroxide. The tyrosin crystals were separated by filtration 
and the filtrate kept again over sulphuric acid. Three crops of tyrosin mix- 
ed with leucin or of leucin alone were obtained in succession. For the 
separation of leucin from the mixture, boiling acetic acid was used. 
Glutaminic acid hydrochloride was separated by saturating the mother- 
liquor from leucin, with hydrochloric acid gas and allowing it to stand in 
an ice box for several days. The yields of the amino-acids follow : 
Tyrosin 0.41 gram; leucin 0.25 gram; and glutaminic acid hydro- 
chloride 0.12 gram. 
Further purification was not attempted because of their small amounts. They 
gave following results on analysis. 
0.1504 gm. tyrosin gave 0.3311 gm. CO 2 and 0.0895 gm. H 2 O. 
Calculated for C9Hn0 3 N=C 59.66; H 6.07 o/ 0 
Found =C 60.04; H 6.61 # 
0.1450 gm. leucin gave 0.2875 gm. CO 2 and 0.1305 gm. H 2 O. 
Calculated for Ce H 13 O 2 X = C 54.96; H 9.92 G /o 
Found =C 54 06; H 9.80 % 
0.1 gm. glutaminic acid hydrochloride gave 0.1201 gm. CO 2 and 
0.0481 gm. H 2 O. 
Calculated for C 5 H 9 O 1 N. HC1 =C 32.69; H 5.45 */ 0 
Found = C 32.75; H 5.35 % 
The presence of other amino-acids is not excluded but the mother-liquor 
from the glutaminic acid hydrochloride was too little to be examined fur- 
ther. 
