530 
Proceedings of the Royal Society 
Fifth Injection. (Grains xxiv.) November 21, 1874. 
Average of 3 days previous to injection, 
First 24 hours after injection, 
Second „ ,, . 
Third „ „ 
Fourth „ „ . 
Total Nitrogen obtained. 
333*36 cubic cents. 
666*38 
763*14 
945*0 
881*9 ,, (Death.) 
The weights of the 
follows : — 
First injection, 
Second „ 
Third „ 
Fourth „ 
Fifth ,, 
these injections were as 
2 lbs. 2 oz. 
2 lbs. 
1 lb. If oz. 
1 lb. 7 oz. 
1 lb. 4 oz. 
animals used for 
In the 6th and 7th injections there was such watery diarrhoea 
that the urine could not be collected separately, and thus the 
results of analysis were not to be depended upon. In the other 
cases the urine was not submitted to nitrogenous analysis. 
It might be stated as an objection, that as the bile acids were 
found in the urine, and as they contained much nitrogen, they 
gave off this nitrogen when treated with hypobromite of soda 
solution, and thus increased the nitrogen of the urine. No doubt 
this is true, but only to a very limited extent ; for when the biliary 
solutions used were separately analysed to ascertain how much 
nitrogen they gave off, it was seen to be so small, that even were 
the whole solution injected excreted during the next twenty-four 
hours, it would be utterly insufficient to account for so great a rise 
in the quantity of nitrogen as was seen to take place after the 5th 
injection. 
Nervous System . — Each injection of bile salts is usually followed 
by drowsiness. In cases which are to end fatally this deepens, and 
the animal at last becomes almost comatose. In this state the 
animal sleeps nearly the whole day, rouses up if touched, but 
merely moves a little, and again falls asleep. Convulsions were 
never noticed, but they might have been overlooked, as the animals 
never happened to be under observation at the time of death. 
