1891 - 92 .] Dr Griffiths on Ptomaines extracted from Urine, 99 
(2) From Diphtheria. 
The ptomaine is also a white crystalline substance. It forms a 
white hydrochloride, a pale yellow aurochloride, a yellow precipitate 
with tannic acid, a white precipitate with phosphomolyhdic acid, a 
yellow precipitate with picric acid, and a brown precipitate with 
Nessler’s solution. 
Analyses of this ptomaine gave the following results : — 
I. 
Found. 
A 
II. 
III.' 
Calculated for 
Carbon, . . 
. 54-21 
— 
54-30 
54-36 
Hydrogen, . 
. 5-56 
— 
5-49 
5-50 
Hitrogen, 
— 
9-00 
— 
9-06 
Oxygen, . . 
— 
— 
— 
31-06 
The above figures correspond with the formula ^14^17^2^6- 
The same ptomaine was extracted from fifteen tubes containing 
pure cultivations of Bacillus diplitherix^ on nutrient agar-agar, 
which had been kept at 36°-37° C. for twenty-one days. The 
method used for extracting and purifying this ptomaine was the 
same as the one which has been already described. 
(3) From Parotitis (Mumps). 
This ptomaine was extracted from urine in a case where the 
kidneys were involved, and the parotid and sub-maxillary glands 
were both affected. It crystallizes in white prismatic needles, 
which are soluble in water, ether, and chloroform. It has a 
neutral reaction and a slightly hitter taste. This base forms a 
yellow crystalline platinochloride, a pale yellow aurochloride, and 
a white crystalline hydrochloride. It combines with phospho- 
molybdic acid, forming a golden yellow precipitate. This ptomaine 
produces a white precipitate with phosphotungstic acid, a slight 
yellow precipitate with mercuric-potassic iodide, a brown preci- 
* Bacillus No. 2 of Klebs and Loffler, which is the same as Klein’s Bacillus 
diplitherioc. 
