106 Proceedings of Royal Society of Edinliirgh. [sess. 
Chemical Characters of the Crystals. — A large quantity of the 
crystals were prepared by decanting the urine, and dialysing the 
residue for several days in sausage-papers with running water, to 
remove urea, salts, &c. The crystals were then taken from the 
dialyser, and repeatedly washed with distilled water by decantation 
to remove all albumin and the remains of the salts. They were 
finally washed with absolute alcohol and ether, and dried over 
sulphuric acid. 
Ash. — The crystals, thus prepared, are practically ash-free ; the 
combustion, at as low a temperature as possible, of 0-965 grm. of 
crystals dried at 110° C. yielded no weighable amount of ash. 
Water of Crystallisation. — To determine the water of crystallisa- 
tion, the crystals were washed with alcohol and ether, and were then 
dried over sulphuric acid at the ordinary atmospheric pressure, until 
the weight was constant. They were then dried at 110° C. until 
the weight again became constant. The percentage loss was, in two 
specimens, 6-01 and 6-6 per cent. 
Griibler found that air-dried crystals of vegetable globulin lost 
10 per cent, at 110° C. 
General Reactions. — The crystals are insoluble in distilled water; 
soluble in dilute solutions of chloride of sodium and sulphate of 
ammonia ; soluble in hydrochloric, in sulphuric, and in acetic acids ; 
soluble in potassic hydrate and ammonia. As the ammonia evapo- 
rates olf, the crystals are sometimes reprecipitated. They are in- 
soluble in hot water and in absolute alcohol. They give the 
xanthoproteic reaction. When heated with strong sulphuric acid, 
they give a reddish colour. They give Liebermann’s reaction with 
hydrochloric acid. They burn with the odour of burned feathers, 
and leave no ash. 
Soluhility. — The solubility of the crystals was tested in sulphate 
of magnesia, chloride of sodium and sulphate of ammonia. 
With chloride of sodium a saturated solution was required to 
prevent solution. In all dilutions the crystals were dissolved. 
In sulphate of magnesia solutions, the crystals were soluble in a 
93 per cent, solution, but insoluble in 100 per cent, solution. 
In sulphate of ammonia the crystals were partially dissolved in a 
16 per cent, solution, and were freely soluble in greater dilutions. 
Temperature of Coagulation. — In solution in the urine, the globulin 
