107 
1891-92.] D. Noel Paton on Crystalline Glohulin. 
commenced to be precipitated at between 59° and 60° C., and was 
completely separated out at 62° C. 
When the pure crystals were dissolved in dilute solutions of 
neutral salts, the coagulation point varied somewhat with the nature 
of the salt employed and with the strength of the solution. 
Sulphate of ammonia, .... 56° to 59° C. 
Sulphate of magnesium, .... 58° ,, 59° C. 
Chloride of sodium, . . . . 58°,, 59° C. 
In all, however, whatever dilution was used, the coagulation point 
was under 60° C. 
The precipitate thrown down on boiling the urine is very 
characteristic. At first the usual milkiness appears, but as the 
temperature rises the precipitate suddenly separates out in a stringy 
fibrin-like mass, which adheres to the sides of the vessel or to the 
rod with which the fluid is stirred. When collected, this fibrin- 
like mass is found to possess a considerable degree of extensibility 
and elasticity. 
The crystals are not precipitated from solution in dilute sulphate 
of ammonia by the addition of acetic acid. 
Composition . — The crystals were dried at 110° C. before they 
were analysed. 
For the determination of the , carbon, hydrogen, and nitrogen, 
I have to thank Mr Murray, B.Sc., who was good enough to perform 
the combustions for me in the Chemical Laboratory of the Univer- 
sity. The sulphur was determined by oxidising with caustic potash 
and nitrate of potash, and precipitating the sulphates with baric 
chloride. 
I believe that this is the first occasion on which a pure crystalline 
globulin from the animal body has been procured and examined. 
