1891-92.] D. Noel Paton on Crystalline Glohulin. 
Ill 
proteids in the urine and in the blood plasma. The present case 
appeared to afford a good opportunity of testing this point. An 
attempt was made to procure a sufficient quantity of blood with the 
artificial leech to allow an analysis of the serum, but was unsuc- 
cessful. 
The patient, who all along showed a deep interest in the scientific 
aspect of his case, accordingly blistered himself on November 15th, 
1890, and on the 16th the blister was opened, and about 2 c.c. of 
serum were obtained. 
An analysis of this showed : — 
Total proteids, . . . . . . 5 '5 
Albumin, . . . . . . . 4’0 
Globulin, . . . . . . . 1’5 
Albumin, ....... 2*5 
Globulin, . . . . . . 
Buchardt has already shown that in such effusions the proportion 
of proteids corresponds to that in the blood serum — the proportion 
of serum albumin being somewhat greater. 
On the same day the urine gave these results : — 
Gravimetric. 
Esbach. 
Total proteids, .... 
1-4 
1-45 
Albumin, 
0-0 
trace 
Globulin, 
1-4 
1-45 
In this case the proteids of the urine showed no relationship to 
the proteids of the blood serum. With the small amount of serum 
at my disposal it was, of course, impossible to investigate the nature 
of the globulin present in it. 
Method . — To estimate the proteids gravimetrically, the total proteids were 
first determined by boiling, and adding a drop of acetic acid and a drop of 
saturated solution of acetate of soda. This brought down a firm ropy pre- 
cipitate, which could readily be collected on a weighed filter-paper washed 
with distilled boiling water, absolute alcohol, ether, and weighed. 
The globulin was precipitated by the addition, to a known volume of the 
urine, of an equal volume of saturated solution of sulphate of ammonia ; 
the mixture was allowed to stand twelve hours, then thrown on a dried filter- 
paper. The proteid of the filtrate, i.e., the albumin, w’as estimated by pre- 
cipitation by boiling, the precipitate being washed frequently with boiling 
water to remove all the ammonia salt, then with alcohol and ether, and 
weighed. The globulin was determined by difference. 
