112 Proceedings of Royal Society of Edinhurgh. [sess. 
To separate the globulin by Esbach’s method, sulphate of magnesia was 
used, since sulphate of ammonia causes a precipitate with picric acid. 
The results by these two methods corresponded closely, as is shown by the 
preceding tables. 
Since the above was written, a paper by Csatary {Deutsche Arch, 
f. Min. Med,, Ed. xlviii. p. 358, 1891) has fully confirmed my 
previous conclusions, and the result arrived at in the present case. 
By an extended series of observations Csatary clearly shows that the 
proportion of albumin and globulin in the urine bears no proportion 
to their relative amounts in the blood serum. 
Metabolism . — A quantitative estimation of the proteids of the 
urine showed that the patient was passing, on many occasions, 
between 60 and 70 grammes of these substances per diem. 
Since this is an amount of proteid which, according to the results 
of the older observers, would be almost sufficient for the daily wants 
of a healthy man, or, according to the more recent results of Hirsch- 
feld and Klemperer, is far more than sufficient, it became a matter 
of importance to study more carefully the changes in the metabolism 
which accompanied this great loss. 
The patient was directed to select for himself a diet to which he 
was to adhere for four days. Each article of diet was roughly 
weighed, and a specimen diet was sent to the Laboratory for exa- 
mination. From this a calculation of the amount of nitrogen taken 
was made. The total urine of each day during the observations was 
sent to the Laboratory, and was examined for the amount of proteids 
and the amount of nitrogen after the separation of the proteids. 
This was done by Kjeldahl’s method. From this the nitrogen in 
the proteids and the total nitrogen of the urine were calculated. 
The following table gives the results of this observation. The 
amount of nitrogen in the food was calculated from diet tables, and 
is approximate. 
These tables show that the patient consumed daily during this 
experiment about twice the ordinary amount of proteid food — 30 
grms. of nitrogen corresponding to 187 '5 grms. of albumin. It 
should be mentioned that during the experiment he was taking 
rather more than his usual diet. 
This large amount of proteid diet was tolerably well absorbed — 
between the nitrogen of the ingesta and that of the urine there was 
