1887.] Dr A. B. Griffiths on Micro-Organisms. 43 
month j and four tubes, containing sterilised blood serum, were 
inoculated from the contents of the tubes. In the two inoculated 
from the open tubes, growths of Bacillus tuberculosis (proved by 
staining and microscopical appearance, &c.) made their appearances 
in sixteen days from the time of inoculation. Growths of Bacillus 
tuherculosis also made their appearance in the two tubes (after being 
inoculated from the contents of the sealed tubes) after nineteen 
days’ incubation. Four more tubes were opened after being 
Fig. 8a. — Bacillus tuberculosis in Acute General Phthisis ; from 
sputum of Mr John Snodgrass, jun. Stained by the Weigert- 
I Ehrlich method, x about 1400. 
exposed for two months at the temperature already mentioned. 
Inoculations from two open tubes revealed the vitality of the Bacillus 
tuberculosis after twenty days’ incubation ; and inoculations from 
the two sealed tubes proved the vitality of the bacilli after the 
elapse of twenty- three days’ incubation. The remaining tubes were 
examined in a similar manner after the elapse of three, four, five, 
and six months respectively. 
