52 
Proceedings of Royal Society of Edinburgh. [dec. 5, 
position of pure sodium bicarbonate by means of pure dilute 
sulphuric acid) and filled a 4-litre bag with the gas. This gas was 
passed through a half-bottle of Eaux Bonnes water (thoroughly 
impregnated with the H 2 S gas), and then allowed to pass into a 
pure cultivation of Bacillus tuberculosis (fig. 10). After all the 
gases had passed through the cultivation, the tap E (fig. 10) was 
turned off. Ten tubes containing sterilised blood serum were 
inoculated from the growths which had been submitted to the 
Fig. 11. — Action of H 2 S and CO2 gases directly upon the bacilli in fresh 
human sputa. A = a drop of human sputum adhering to the cover- 
glass. 
action of the gases. The tubes so inoculated were then placed in 
the incubator at a temperature of 37° C. After forty days’ incuba- 
tion, no signs of any growths made their appearance in any of the 
Fig. 12. — Bacilli in Sputum. Case of Miss Green- 
White. Stained by the Koch-Ehrlich Method. 
X 750. 
tubes. These experiments were repeated a second time with similar 
results. 
Again, the gaseous antiseptics were allowed to pass for 15 minutes 
