PROSTATE GLANDS OF THE EARTHWORMS OF THE FAMILY MEGASCOLECID^E. 439 
The specimens of Eutyphceus waltoni Michlsn., a common species in the United 
Provinces, were those prepared and fixed by Professor Younuman, of Canning 
College, Lucknow, as mentioned in the following paper. We also used specimens 
obtained by one of us at Agra, and fixed in Zenker’s fluid. 
The specimens of Dichog aster malayana (Horst) and D. a finis (Michlsn.), from 
Negyatankaray in Travancore, and Anuradhapura in Ceylon respectively, belonged 
originally to the Indian Museum, and were sectioned in the course of systematic 
work done on the collections in which they occurred. They were not specially fixed 
for histological work, and hence have not been described in greater detail than 
is warranted. 
The examples of Ocnerodrilus ( Ocnerodrilus ) occidentalis Eisen were found near 
Lahore some time ago, and had been fixed in Zenker’s fluid. 
For staining, Delafield’s haematoxylin followed by eosin gave on the whole the 
most useful results, the granules in the glands being stained differentially ; and most 
of the descriptions are written and drawn from specimens so prepared. Heidenhain’s 
iron-haematoxylin in Dobell’s modification, brazilin, van Gieson’s stain, and borax 
carmine followed by picroindigocarmine were also used. 
Pheretima hawayana. 
(l) Structure of the Gland as seen in Dissection. 
On dissection (fig. l) the gland is seen to take up segments xviii, xix, and xx ; it 
is tripartite, being constricted at the level of septa 18/19 and 19/20, which get an 
attachment to both inner and outer faces of the gland. Each gland is flattened 
between intestine and body- wall, and is situated laterally and dorsolaterally, extend- 
ing dorsalwards to not very far from the mid-dorsal line. 
The surface of the gland is marked out into a number of small closely apposed 
lobules, which, however, are not very distinct ; the edges of the gland are slightly 
indented, in correspondence with this lobulation. 
A thin sheet of connective tissue, attached at both ends to the body-wall, and 
somewhat triangular in shape with its base internal, binds down the antero-internal 
angle of the gland to the body- wall. The wider end of this sheet of tissue is just 
underneath the edge of the intestine (as the parts are displayed in dissection) and 
internal to the termination of the prostatic duct ; its narrower apex is at the level 
of and rather underneath the antero-external corner of the gland. It is interesting 
to compare this band of tissue with the connective tissue investment of the penial 
setal bundle in Eutyphoeus ( cf. pp. 447, 448) ; both are attached above not far from 
the mid-dorsal line, and below internal to the ending of the prostatic duct ; both are 
narrower above than below ; and both bind down the gland to the parietes. Though 
penial setae are not found in Pheretima , they are present in many of the more 
primitive genera of the sub-family (Megascolecinae) to which Pheretima belongs. 
