of Edinburgh, Session 1885-86. 
617 
“ vaccine ” contain probably, not micrococci, but the spores of 
micrococci. Inoculation with these materials produces local irrita- 
tion by the growth of the “ spore ” into a more mature form of 
organism. 
3. Opaque vaccine lymph and opaque variolous lymph contain 
larger, more refractive bodies of various sizes, which are probably 
developed from the spores in clear lymph. They increase in size 
and number in proportion to the length of time the lymph has been 
kept. After a few months large torulse crowd the field in micro- 
scopic preparations. Such lymph has an acid reaction, and when 
inoculated it produces either violent local action, i.e., accelerated 
vaccination, or we may have no local action, but secondary eruption 
in some other part of the body. This is probably due to rapid 
spore-production by the torulse. The spores are probably absorbed 
into the blood, and rapidly grow there, until they are arrested in the 
capillaries, where they continue to grow and set up irritation ending 
in the production of vesicles or pustules. From these secondary 
foci may again be produced. This would account for the appear- 
ance of periodic secondary eruptions after vaccination. The growth 
from the spore to the torula explains the occurrence of opacity in 
lymph. Drying the lymph prevents this growth, and also deteriora- 
tion of the true vaccine material. 
4. White, yellow, and dull ochre or orange cultivation of vaccine 
lymph in solid media, appear to represent stages of growth between 
the spore and the torula. They correspond to the refractive cor- 
puscles and cells described by Cohn in vaccine lymph. When 
inoculated, they produce in calves eruptive fever. They do not 
produce a local result. 
5. White variolous cultivations in solid media appear to represent 
stages of growth between the spore in clear variolous lymph and 
the torula in opaque variolous material. When inoculated, a mild 
eruptive fever was produced in a monkey. This was not contagious 
to another monkey. The organisms correspond to the refractive 
corpuscles of Cohn. Cultivations of variola and vaccinia in solid 
media appear to produce mild eruptive fever. They do not produce 
a local result. 
6. Inoculation with vaccine and variolous cultivations protects 
from a subsequent attack of variola in calves. 
