of Edinburgh, Session 1885-86. 
773 
readily enough. At this period or later the character of its jump 
becomes altered ; there is a very decided stiffness in its movements, 
and when it jumps it lands rather heavily on its belly, the hind 
legs being somewhat straggling. The unwillingness to move in- 
creases, and the frog, when irritated, walks rather than jumps. In 
walking the gait is peculiar, the limbs being stiff and almost fully 
extended. The pupil is always dilated either at this stage or later. 
In about eighteen to twenty-four hours after administration the 
muscles are still in the same condition, hut the reflex excitability 
has in the interval become very much increased. The extent of the 
increase varies, the animal being in some cases thrown into a state 
of violent tonic spasm, exactly resembling that induced by strychnia, 
and in other cases simply showing greatly exaggerated reflexes. 
When the reflexes are only slightly affected respiration and circula- 
tion go on as usual. 
This condition lasts one or two days, the state of greatly increased 
reflex excitability gradually passing off, hut the skin reflexes remain 
exaggerated for one or two days longer, at the end of which time 
the muscular stiffness has also disappeared, and the frog returns to 
its normal condition. The time required for complete recovery 
varies in different frogs. 
Doses of J to 1 grain (0 , 032 to 0064 grm.) always proved fatal. 
Shortly after the injection the animal became very dull and un- 
willing to move. It could still jump, and did so as previously de- 
scribed, hut in a more pronounced manner. In about eighteen hours 
tetanus supervened, lasting until death from general exhaustion 
occurred, usually about the third day. Respiration goes on inter- 
mittently, and the heart continues to heat until the exhaustion is 
very marked. 
Post-mortem Appearances . — Heart in diastole and not excitable 
by electric stimuli. Ventricle is acid in reaction, and usually has a 
peculiar shrunken look. 
The veins are filled with venous blood, and the viscera generally 
are very congested. 
The skeletal muscles at the point of injection did not contract to 
electric stimuli. The other muscles generally contracted at 60 to 70 
mm. (Du Bois Reymond induction apparatus and one Daniel’s cell), 
and had a strongly acid reaction. 
