of Edinhurgh, Session 1883-84. G37 
spores ; any growth, or turbidity was submitted to microscopical 
examination. 
A few experiments were made after the manner of Koch on 
nutrient gelatin, but it was not found so convenient as fluids. 
Although the microscope was in each case employed, I think all 
those who have worked at the subject will agree with me, that in 
perfectly normal cults, a little practice enables the anthrax cult to 
be distinguished by the naked eye from all other cults whatever. 
Cay'holic Acid. — The carbolic acids experimented upon were Cal- 
vert’s Ko. 1, and the dark carbolic acid of commerce, containing tar 
oils and much foreign matter ; Calvert’s carbolic acid powder was 
also tested. 
Calvert's Carbolic Acid No. 1. — A 1 per cent, solution in water 
of this acid was made, and the anthrax spores soaked in it, in the 
way detailed and then cultivated in the pork broth. 
Time during wliicht he glass rod 
with its spores rested in the Result, 
disinfectant. 
1 per cent. 10 minutes. Active growth in twenty- 
four hours. 
„ 1 hour. do. 
„ 24 hours. do. 
Hence 1 per cent, of carbolic acid seemed to have no influence on 
anthrax spores, not even delaying the growth, for no decided differ- 
ence between the disinfected cult and a cult not disinfected could 
be distinguished. 
Experiment with 5 per cent. Solution of Carbolic Acid. 
Time during which the glass rod 
with its spores remained in the Result, 
disinfectant. 
10 minutes. 
20 minutes. 
4 hours. 
After twenty-four hours, growth evident, 
but not anything like so plentiful as 
in the “ control ” flask ; in four days 
growth luxuriant. 
Do. 
Ko growth in twenty-four hours ; after 
forty-eighyiours, slight development; 
in four days a good growth. 
