638 
Proceedings of the Boycd, Society 
Experiments with a 10 per cent. Solution. 
Time during wliich the glass rod 
with its spores remained in the 
disinfectant. 
Result. 
20 minutes. 
The “fluff” was freed by alcohol from 
the adhering film of carbolic acid; 
growth was delayed, but by the end 
of four days there was a good 
growth. 
4 hours. 
No growth in twenty-four hours ; after 
forty-eight hours there was an ap- 
pearance of some of the spores being 
active; in a few days a scanty growth 
was noticeable. 
24 hours. 
No growth. 
Experiment ivith a 25 per cent. Solution in Alcohol. 
Time during which the glass rod 
with its infected “fluff” re- Result, 
mained in the disinfectant. 
Dr Klein kindly experimented with my 10 per cent, solution on 
a guinea-pig; but although I wns unable to cultivate the spores 
successfully after twenty-four hours’ soaking, the guinea-pig died of 
typical anthrax poisoning in two days. 
Experiment with the Undiluted Acid. 
Spores soaked for twenty-four hours in the undiluted acid did not 
afterwards develop. 
Cresylic Acid. — A 25 per cent, solution of cresylic acid in alcohol 
acting on anthrax spores for twenty-four hours only delayed the 
growth. On the “fluff” being placed in a sterilised broth, there 
was no turbidity for twenty-four hours; while in the “ control” the 
growth was evident and strong ; afterwards a few threads slowly 
developed. By the end of four days the liquid was quite turbid. 
Similar results were obtained by cultivating on “gelatin-pork.” 
6 hours. 
After twenty-four hours some de- 
velopment, and in four days a 
good growth. 
24 hours. 
No growth. 
