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Proceedings of the Royal Society of Edinburgh. [Sess. 
origin of the sympathetic chain from the intervertebral ganglia. This 
description differed from that given by Onodi of the same structure, in the 
fact that His regarded the cells of the future sympathetic chain as existing 
in an immature condition in the intervertebral ganglia, and only attaining 
maturity when they reached the position of the future sympathetic chain. 
As regards the visceral ganglia, they were described in Onodi’s paper as 
originating from the sympathetic chain. 
In 1890 also appeared a paper by His junior and Romberg (12), in 
which an account was given of the ganglionic cells in the heart, and of 
their relation to the sympathetic chain. 
In 1891 His junior (13), in a second paper on this subject, gave a 
detailed description of the route followed by the nerve elements in their 
migration from the sympathetic chain to the heart. 
In 1897 His junior (14) published a detailed description of the develop- 
ment of the abdominal sympathetic in the chick. A description was given 
of observations made from the third to the tenth days of incubation. 
At the end of the third day sympathetic nerve cells were recognised in 
the cervical portion of the chick lying behind the carotid, and in the 
thoracic and abdominal portions behind the aorta. The recognition of 
these cells was based on their size, which was described as larger than the 
mesoblasts, and on the fact that their reaction to haematoxylin and eosin 
(the stain used) was better marked than that of the mesoblasts. Between 
these nerve cells and the spinal ganglia loosely arranged groups of nerve 
cells were recognised. At this age a very fine band of nerve tissue was 
described in the lowest portion of the intestine. From its character and 
position it was recognised as the intestinal nerve of Remak, but at this 
stage no actual connection was demonstrated between it and the rudi- 
mentary sympathetic chain. But His regarded it as undoubtedly the 
result of a cell migration from that structure. 
At the end of the fourth day the sympathetic chain was found to be 
well advanced in development, and well-marked cellular migration from it 
was seen at the level of the heart. The intestinal nerve of Remak was 
easily recognised in the lower intestine, while in the stomach the vagus 
branches appeared. 
At the fifth day sympathetic nerve cells were found in the stomach and 
intestinal wall. At the pyloric end of the stomach the first signs of a 
double layer of nerve tissue were seen. At this age a connection was 
found well established between the intestinal nerve of Remak and the 
sympathetic chain. 
At the sixth day the secondary sympathetic chain described by His 
