475 
1907-8.] On the Effects on Metabolism of Chloroform. 
The Ammonia Nitrogen was determined by Schloessing’s method. 
The Creatinin Nitrogen , when investigated, was determined by Folin’s 
method. 
The Uric Acid Nitrogen in the early experiments was determined by 
Hopkins’ method, and in the later experiments (XI. and XII.) by Folin’s 
method. 
The Oxidised and Unoxidised Sulphur were determined by the method 
described in Jour, of Physiol., vol. xxiv., p. 335, 1899. 
Most of the determinations of nitrogen were carried out under my 
supervision by Mr Alfred Patterson, Chemical Attendant in the Royal 
College of Physicians Laboratory. The sulphur determinations were made 
by Miss Jean Robertson, Chemical Assistant in the Laboratory. 
IV. — Results. 
Series I. — Chloroform by the Respiratory Passages. 
The chloroform was administered upon cotton wadding placed in a mask. 
Since several determinations have now been recorded of the chloroform 
in the blood during full anaesthesia, showing that it amounts to something 
not far short of 50 mg. per 100 c.c. (Grehant and Quinquand, C.R., 1883 ; 
Nicloux, C.R. Soc. biol., t. lx., p. 244 ; Buckmaster and Gardner, Proc. Roy. 
Soc ., vol. Ixxix., 1907), it was not considered necessary to dose the amount 
given. Generally in two hours about 80 c.c. were used. 
Experiment I. — Retriever Bitch. Weight, 16 kilos. 
o ' 
Table I. 
Day. 
Urine 
in c.c. 
Sp. G. 
Total 
Nitrogen. 
Urea Nitrogen. 
Bohl* jM.&S.t 
nh 3 
Nitrogen. 
1 
880 
1013 
6-22 
5-32 
0-579 
2 
910 
1013 
4-96 
0-403 
3 
620 
1013 
4T2 
0-299 
4 
690 
1014 
4’06 
3*39 
0-274 
5 
220 
1025 
2*77 
2-41 
2-27 
0-126 
Fast. 
6 
640 
1010 
3-95 
333 
3’11 
0-252 
7 
570 
1013 
3-30 
2*86 
2-66 
0-224 
8 
770 
1018 
6-94 
5-63 
5-68 
0-512 
9 
690 
1015 
5-48 
4-68 
4-56 
0-397 
Fast. Chloroform 1 hour. 
10 
770 
1014 
4-87 
4*26 
4-01 
0229 
11 
690 
1012 
4-09 
3-50 
3*33 
0-319 
Weight, 15 kilos. 
No protein and no reduction of Fehling’s solution. 
* Bohl. = Bohland’s pliospho-tungstic acid method, 
t M. & S. =Morner and Sjoqvist’s method. 
