487 
1907-8.] On the Effects on Metabolism of Chloroform. 
1. Total Nitrogen. — These experiments show the very marked effects 
of chloroform administered by the stomach upon the protein kata holism. 
In both, a very markedly increased excretion of total nitrogen followed the 
administration of the substance. 
2. Urea Nitrogen. — A very marked fall in the proportion of urea 
nitrogen, as determined by Morner and Sjoqvist’s method, is manifest in 
each experiment, reaching its maximum on the third day. In Experiment 
VII. it amounts to 17 per cent., and in Experiment VIII. to 13 per cent. It 
is to be regretted that the conformity of the results obtained by the two 
methods of determining the urea nitrogen in the previous series of experi- 
ments decided me to employ one only in the present set. 
3. Ammonia Nitrogen. — A rise in the proportion of ammonia nitrogen 
accompanies the fall in urea nitrogen, but the maximum of the one does 
not always occur on the same day as the minimum of the other (figs. 1 
and 2, p. 493). In both experiments the maximum proportion of ammonia 
nitrogen is reached two or three days after the minimum proportion of 
urea nitrogen. 
4. Creatinin Nitrogen. — The total excretion of creatinin nitrogen in 
these experiments follows fairly closely the excretion of total nitrogen. 
Hence the proportionate excretion does not undergo very marked varia- 
tions, but in both experiments the rise in creatinin nitrogen is not pro- 
portionate to the rise in total nitrogen on the day after the administration, 
and hence the proportion falls somewhat. 
5. Uric Acid Nitrogen. — In both experiments there is an increase 
in the total amount of uric acid nitrogen excreted, but this occurs 
earlier, on the day following the chloroform, in Experiment VII., and later, 
three or four days after, in Experiment VIII. In Experiment VII. the 
proportionate increase appears on the day after the administration, and 
in Experiment VIII. not until the third day and after. 
6. The Nitrogen not in Urea and Ammonia , and the nitrogen not in 
urea, ammonia, creatinin, and uric acid, show a rise after the administra- 
tion of chloroform. 
7. Sulphur. — The excretion of sulphur in Experiment VII. runs parallel 
with the excretion of total nitrogen. The proportion of oxidised sulphur 
is decreased after the administration of chloroform, but is not lowest 
on the day of the lowest proportion of urea nitrogen. 
In both experiments, proteins, granular cells, and tube-casts were 
present in the urine. There was no reduction of Fehling’s solution. In 
Experiment VIII. bile-pigments appeared in the urine, and the animal was 
slightly jaundiced. 
