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Proceedings of the Royal Society of Edinburgh. [Sess. 
V. — Consideration of Results. 
These experiments show that in the dog chloroform, when given by the 
respiratory g>assages, for two or three hours has either no effect upon the 
nitrogenous metabolism or increases the protein disintegration, as already 
demonstrated by previous investigators. The effect upon the nitrogen in 
urea was either to leave it unaffected or to increase its proportion, while, at 
the same time, the nitrogen in ammonia was either unchanged or decreased 
when the urea was increased. These results suggest that the hepatic 
metabolism, by which ammonia compounds are changed to urea, is stimu- 
lated — an effect very similar to that produced by the administration of a 
protein diet (Jour, of Phys., vol. xxv., p. 443, 1900) — and that this stimu- 
lation, in these experiments at least, is not followed by any after-depression. 
The toxic action of the drug upon the kidneys is manifested by the 
presence of proteins and of renal epithelium in the urine, most markedly in 
Experiment II., where the animal was ansesthetised for two hours, and again 
for one hour. 
When chloroform is given by the stomach the effect is very different. 
The protein disintegration, as indicated by the excretion of nitrogen, is 
markedly increased, and this increase is best marked on the day of, or the 
day after, the administration of the drug. At this time the disturbance in 
the proportion of nitrogen in the various compounds is not very pronounced, 
and the presence of protein in the urine is not so distinctly marked as it 
afterwards becomes. There is apparently a period of simple increase of the 
protein disintegration. 
After two or three days there occurs a very marked fall in the 
proportion of nitrogen in urea, with a rise in the proportion of nitrogen 
in ammonia — a rise which, however, is not inversely proportionate to the 
fall in urea nitrogen, and which is somewhat delayed (figs. 1 and 2). Along 
with this there is a rise in the proportion of uric acid nitrogen, which 
apparently may precede (Experiment VII.) or succeed (Experiment VIII.) the 
most marked fall in the urea nitrogen. The proportion of nitrogen not in 
these analysed compounds increases concomitantly with the fall in the urea, 
and previously to the rise in the ammonia. In both the experiments the 
rise was a very marked one, corresponding to something like 6 per cent, 
of the total nitrogen. Since the urea nitrogen was determined by the 
method of Morner and Sjoqvist, the amino acids were precipitated, and not 
estimated with the urea, and hence almost certainly this increase in the 
undetermined nitrogen is due to the appearance of amino acids. It is to 
be regretted that, since the previous examination of the urine after the 
